Nucleobase Transport

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The discovery and characterization of novel nucleobase transport proteins. Purine and pyrimidine nucleobases are essential precursors for the salvage of nucleotides and can act as important signaling molecules. While it has been established that nucleobases can selectively and actively traverse cell membranes, the mechanism of this transport process is still unknown. Our lab is developing bi-functional nucleobase probes to selectively tag and identify membrane-associated nucleobase binding proteins. We are conducting this work in collaboration with the lab of Yana Cen at Albany College of Pharmacy and Health Sciences. We have also developed a novel colorimetric proteoliposome-based assay to characterize nucleobase transport kinetics and selectivity.